中文版
English应用:
生化诊断
产品类别:
酶
生化诊断:
比色法
产品咨询
产品描述
规格
| Appearance | yellow lyophilizate |
| Activity | ≧4.0 U/mg |
| Stabilizer | trehalose |
| Storage condition | below -20℃ protected from light |
特性
| Molecular weight | ca. 45 kDa (gel filtration) |
| Structure | monomer of 50 kDa (SDS-PAGE) |
| Michaelis constant | 2.2×10-4M (Nε-fructosyl-L-lysine) |
| pH Optimum | 8.0–8.5 |
| pH Stability | 6.0–8.5 |
| Optimum temperature | 35–40℃ |
| Thermal stability | below 30℃ |
| Stability (liquid form) | stable at 25℃ for at least two weeks |
| Stability (powder form) | stable at 37℃ for at least three weeks |
| Inhibitors | Ag+，Cu2+ |
| Specificity | Nε-fructosyl-L-lysine (100), fructosyl-L-valine (65) |
| fructosyl-glycine (30) |
应用
The enzyme is useful for the determination of fructosyl-L-amino acid.
参考文献
Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).
Sakaue, R. et al., Biosci. Biotechnol. Biochem., 66, 1256–1261 (2002).
Hirokawa, K. and Kajiyama, N., Biosci. Biotechnol. Biochem., 66, 2323–2329 (2002).
Sakaue, R. and Kajiyama, N., Appl. Environ. Microbiol., 69, 139–145 (2003).
