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60233

所属分类
Kikkoman
中文名称:
麦芽糖磷酸化酶
英文名称:
Maltose Phosphorylase
The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.
应用:
生化诊断
产品类别:
生化诊断:
比色法
产品描述

规格

Appearance white lyophilizate  
Activity ≧10 U/mg  
Stabilizer lactose, ethylenediaminetetraacetic acid (EDTA)  
Storage condition below -20℃  

 

特性

Molecular weight

ca. 220 kDa (gel filtration)

Structure 2 subunits of 90 kDa (SDS-PAGE)
Michaelis constant

1.9×10-3M (maltose)

3.4×10-3 M (phosphate)

8.3×10-3M (arsenate)

pH Optimum 6.5–7.5
pH Stability 5.5–8.0
Optimum temperature 45–50°C
Thermal stability below 55℃
Stability (liquid form) stable at 37℃ for at least one week
Stability (powder form) stable at 30℃ for at least four weeks
Inhibitors

Hg2+,Ag+,Zn2+,Cu2+

 

应用

The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

 

 

参考文献

Hiruma, M. et al., Nippon Nogeikagaku Kaishi, 70, 773–780 (1996).

Shirokane, Y. et al., Carbohydr. Res., 329, 699–702 (2000).

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